Backpedaling

In describing nature, whenever we use absolute language, and we do that a lot, we have to backpedal or face clear deficiencies in our descriptions and contradictory data.

Karl Jaspers said that one has not understood what Nietzsche wrote until one also finds the contradiction. Is this unique to Nietzsche? I doubt it.

In the popular picture of Hegel’s thought, thinking correctly moves from thesis to antithesis to (Hegelian) synthesis. Indeed, if one proposes an absolute thesis, one has to deal with contradiction. Hegel’s way was a type of syncretism.

How does one say something meaningful without having to backpedal? Answer: by eliminating all absolute words and terms and any words or terms that can be confused with such. We need a computer program that does this and suggests properly worded alternatives. The goal is no backpedaling. Clarity is truthful descriptiveness of nature and that implies no backpedaling.

Another useful program: one that extracts contradictions and puts contradictions and absolute statements on facing pages. I would love to write this program to highlight the backpedaling that so many people ignore – and to catch my own before I have to backpedal.

A useful invention

Lactose-free milk – milk that has had the lactose removed, substantially or completely.

Another version is ‘sweetened’ with something that is not convertible into pyruvate. Sweet proteins, sweet amino acids, even sweet sugar alcohols.

What we have is lactase-treated milk – glucose and galactose replaces lactose, too much sugar.

The invention would be useful for cancer patients and patients with degenerative disease generally. Sugar feeds tumors. Sugar maintains the degenerative lactic acid production in cells with impaired, defective, or slow mitochondria.

Milk without lactose would have fat and protein, without sugar – good for normal cells, bad for cells dependent on the conversion of sugar to lactic acid, bad for degenerative, dedifferentiated cells, bad for cancer cells,  which are not as metabolically flexible.

Great for Atkins’ dieting, too, a highly nutritious, delicious tasting liquid without carbohydrate. Extra fat could be added to make it taste even more delicious! A dream!

As we age, we need more parenteral nutrition

As we age, the efficiency of both our digestion of food and the absorption of nutrients from the digested food declines. Doubtless, so does the efficiency of our cellular uptake of nutrients from the blood stream (and not just because of lower concentration of nutrients in the blood due to poorer digestion and absorption), a problem that even parenteral nutrition won’t fix.

Parenteral nutrition would get us past the digestive and absorption inefficiencies. I’m thinking of regular self-administered shots of nutrients, not an IV hookup.

We also need an invention to get us past the inefficiencies of cellular uptake from the blood stream. Something that generically pushes much-needed nutrients into cells, and then deep inside them to the subcellular compartments. As we age, I wonder whether our mitochondria are even getting all of the oxygen that they need, or whether our cells are even getting all of the water that they need.

Needful things – Part 1

1. A removable, washable, comfort sweat-band for hats. Could be velcroed onto the hat. It -not the hat- absorbs the sweat. Keeps the hat from looking bad (sweat lines are so damned obvious). Keeps the forehead from getting repeatedly microbially (bacteria, viruses, and molds) contaminated from putting on a dirty hat.

What an absurd protocol we follow! What are we thinking of!

Improved nutritional supplements

Nutritional quality is measured by the total nutrient / calorie ratio.

Nutrients include essential and V3 (molecules involved in the essential nutrient “complex” or network).

Most supplements are deficient in V3 nutrients and seriously unbalanced in the ratios of essential nutrients.

Use whole foods as a starting material to get better balance and more V3 nutrients, and remove as many calories as is feasible, given that nutrient integrity is maintained.

For example, make a puree of mixed fruits and vegetables, remove some of the fiber, and most of the sugars and starches as is practical, remove the water, and make into pills or powders. The powders could be added to foods like smoothies to increase the whole foods’ nutrition by increasing total nutrition/calorie.

Improved quantitative branched DNA hybridization assay by Mark L. Collins

  1. Simplify manufacturing by limiting the number of capture extenders (CEs) and label extenders (LEs) to 2 in most cases. 4 total probes should provide fairly uniform hybridization efficiency to highly structured targets. This limited number of extender probes would also make the bDNA assay easier to multiplex and to manufacture.
  2. The multiplex assay would optimally be done on beads with different capture sequences, followed by separating beads based on specific capture sequences or tags correlated to specific capture sequences, then specific dehybridizations (optional, but recommended), then detection.
  3. Computer design the CE and LE probes to absolutely minimize cross-hybridization to each other and to the generic sequences.
  4. Screen each CE against each LE probe and choose probes with highest signal {target present}/noise {no target present}. This screen should also remove LE probes with homology to generic capture probe.
  5. Tail the label extenders with 3000-5000 residues of dC. Purify.
  6. Tail oligo(dG)-oligo(dA) with 3000-5000 residues of dA to make a generic preamplifier. Purify
  7. Hybridize the LEs and CEs to the target in the microwells overnight.
  8. Wash stringently.
  9. Hybridize the preamplifier to the targets bound in microwells
  10. Wash well.
  11. Hybridize the generic branched DNA to the generic preamplifier (the bDNA has a dT20 sequence in it, which will hybridize to the poly(dA) tail of the preamplifier.
  12. Wash well.
  13. Bind labeled probe to branched DNA amplifier.
  14. Wash well.
  15. Detect with most sensitive method, e.g. chemiluminescence.
  16. Alternatively, de-hybridize with a sequence complementary to the well-bound capture probe at relatively low stringency in chemiluminescent detection reagent.
  17. Transfer to blank opaque wells and detect (should enhance S/N by leaving a lot of background behind).
  18. The amount of signal amplification per LE could be as much as 500 * 250 * 45 = 5, 600,000. Using dC5000, dG10-dA5000, dT20 with a branched DNA with 45 sites for binding labeled probe.
  19. 1-10 molecule detection should be routine.
  20. Improvement: bind the chemiluminescent enhancers directly to the target via hybridization of another type of LE for a smarter, more target-dependent signal.

A good and useful invention

I wish someone would invent a filter that could be applied to print, video, etc. It would select for scenes in which a designated character or characters appear. Or a scene in which something occurs. Etc.

For video, the video would be loaded with an index of all scenes. Selecting for those scenes is just a matter of looking-up the selected items, selecting them, and playing them in sequence, or randomly if one so desired.

For example, all of my favorite scenes in “Doc Martin” are scenes with Louisa in them. So I would select all episodes of “Doc Martin” and then select “with Louisa” and the program would create a montage of the entire series with every scene in which Louisa appears. Would love it! The program would replay all of my favorite scenes.

My second favorite set of scenes are those in which other characters are talking about Louisa. Again, there would be a set of these scenes, which could be loaded based on an appropriate scene descriptor.