What would we do if we had a floppy pyruvate dehydrogenase structure? How about eating properly and thereby getting enough potassium? Potassium stabilizes the floppy loops in pyruvate dehydrogenase and a whole lot of other intracellular proteins.
What would we do if we have a floppy enzyme that is overproducing free radicals? We would probably start eating right, and our diet would contain lots of potassium, taurine, and other protein structure stabilizers, and the problem would more or less go away. We might also supplement appropriately and complementarily, as no doubt other substances do the same thing (e.g., zinc and tetrahydrobiopterin help stabilize the structure of NOS so that it does produce more NO and does not produce so much superoxide).
Fix obvious deficiencies in all small molecule protein structure stabilizers (like protein loop stabilizer potassium and taurine osmolyte) if a mutated p53 is a little too floppy long before adding toxic drugs. Sorry that potassium and taurine do not have pots of gold attached to their use.
- When the mutant p53 has a mutated zinc binding site and does not bind zinc very well.
- More generally, nature uses a mixture of protein denaturants and protein stabilizers to achieve the right balance. One must never forget the importance of the appropriate activities of the protein denaturants as well. There is no substitute for eating right and no excuse for wasting calories on junk.