Mouse experiments show that mice on a chemically defined diet (i.e. no real, no whole foods) containing 35% purified soybean oil develop atherosclerosis much more rapidly than those on the standard low fat mouse diet.
The first missing control: high fat + all of the components in fat’s DADME network, consisting of every compound that in any way aids the proper digestion, proper absorption, proper distribution, proper metabolism, and proper excretion of fats or fatty acid metabolites.
The second missing control: a serious attempt to model a human high fat diet that contains olive oil, milkfat, canola or soybean oil, beef fat, chicken fat, pork fat, and others.
The third missing control: mice fed real food, standardized to contain optimal levels of the known mouse essential nutrients.
Without at least the first control we cannot tell whether high fat is atherogenic for mice or if a deficiency in fat’s DADME network leaves them vulnerable. Extrapolations to human beings is problematic in any case.
Epidemiological studies on Americans are flawed by not defining a control cohort of properly nourished individuals, who have sufficient levels of all of fat’s DADME network nutrients. These individuals would be key in studying the effect of diets higher in fat on the risk of developing atherosclerosis.